Microscopy Geeks: Cytoplasmic Streaming?

[OkRad]

I have heard 2000x is best for cytoplasmic streaming. Yes? This would be for plants. I guess blood drops would be fun, too! But mostly to see cytoplasmic streaming in plants.

 

[Ronald Zeeman]

A light microscope is limited too 900X higher than this go need an electon microscope.

 

[Gerald Wilgus]

Used to do a lot of microscopy. One project was to measure cell cycle length and S-phase duration in rat mammary gland epithelial cells. I did this through dual labeling with tritium or C-14 Thymidine. S-phase is calculated from the time Between tritiated Thymidine and C-14 Thymidine injections and the proportion of cells with single and dual labels. The tissues were fixed, sliced by microtome, stained, and then coated with a photo emulsion and allowed to develop. Then a Collodion layer was applied, to stop the alpha from the tritium and another photo emulsion applied then developed. One could then see single or dual labels by focusing through the two emulsion layers since C-14 beta would expose both layers, and Tritium alpha only the lower layer, then tally their proportions.

 

[Ronald Zeeman]

A bit more involved than What I did in college gram staining, looking under the microscope, but then engineering was more my interest.

 

[Yeshuasdaughter]

I don't know much about this subject. But I do love looking in microscopes. It was wonderful teaching biology last year, because my daughter and I got to spend a lot of time magnifying different specimens under the scope.

It was fun being able to see stuff like the glucose in leaf veins and the different things that owl pellets were composed of.

Spoiler

And don't even get me started on my fascination with binoculars or telescopes!

 

[OkRad]

They have these contraptions you can hook up to a phone and see stuff, supposedly up to 2000x. I am mostly interested in seeing streaming in leaves, chlorophyll movements. Next semester is heavy bio so I hope to do more with actual lab stuff!

Here is one: All fake or any good?

https://www.amazon.com/Oxbird50X-20...cphy=9010065&hvtargid=pla-1135947826952&psc=1

 

[Ronald Zeeman]

Any thing that says you can magnify greater than 900 times with a visible light microscope is fake. The laws of physics has not changed since I was in college.

 

[Crossbreed]

Let's Get Small, Steve Martin (1977)​

 

[watersprite]

You can see individual blood cells at 1000x.

 

[Gerald Wilgus]

Any thing that says you can magnify greater than 900 times with a visible light microscope is fake. The laws of physics has not changed since I was in college.

Exactly. The limit to oil immersion optics with visible light is 1,000 x, and that is also assuming you are coupling the condenser to the specimen with high refractive index oil.

 

[Ronald Zeeman]

Your right I forgot about the oil it has been over 40 years.